Chromatogram Trace Viewers
These programs are limited in scope but serve a good purpose in evaluating the quality of data produced by allowing users to view the raw data associated with .ab1 files.
FinchTV (Windows/OSX)
From the makers of our data management system. Displays quality scores and much more. We really like the feature that links directly to NCBI for a BLAST search! What is more the application is FREE!
To get your free 15-day evaluation license or to update your version of Sequencher to 5.4.6, follow these four easy steps. This will provide you with the full Sanger and NGS functionality for your DNA Sequencing. Step 1: Download. Step 2: Download. Note: GSNAP is 64-bit only in Sequencher 5.4. Protein sequences can be reverse translated into DNA, compared using 'Dot-Plot' analysis and scanned for Proteolytic cleavage sites and amino acid sequence motifs. A comprehensive Protein Analysis Toolbox provides a wide variety of algorithms for analyzing the composition of proteins and presenting the results in graphical and tabular formats.
4Peaks (OSX Only)
Written to run natively on OSX and makes full use of the OSX interface. The application has lots of features and is more than just a “chromatogram viewer”. The best feature is the application is totally FREE! The same people have other OSX applications that might be useful for scientists.
ABI Seq Scape(Windows)
Another free viewer. This program is provided by the maker of the machine we run your samples on.
Chromas LITE (Windows Only)
This application will allow PC users to display, edit and print Applied Biosystems chromatogram files. You can even translate the sequence and reverse complement it! There is a ‘Pro’ version with more features.
Traceviewer(Windows/OSX/Linux)
This is a Java program that allows the reading of sequence traces from ABI or SCF files, the display of traces on white or black background and the display of Phred quality values as bars or numbers with one of three different color schemes. TraceViewer is free for academic users. Traceviewer for the Macintosh does not suffer from the same problem as Editview.
Contig Assembly and Sequence Alignment
These software packages can be used to view, edit, assemble, align, BLAST and even design primers. Some of the programs have phylogenetic analysis software built in as well! For more information, click on the links below.
Proprietary Software:
Proprietary software may cost more, but it also tends to be more current and offers long term and ongoing support.
- CLCbio DNA Workbench (Windows/OSX/Linux) Available for use on workstations in our facility
- Bioinformatics Toolbox 3.1 for MATLAB (Windows/OSX/Linux)
- CodonCode Aligner (Windows/OSX)
- Geneious(Windows/OSX/Linux)
- iNquiry (OSX Server/Unix/Linux)
- Lasergene (Windows/OSX)
- Sequencher (Windows/OSX) v4.2 available on Mac workstations in our facility
Free and Open Source Software:
Free, customizable and often times you can examine the source code. These can also be difficult to find support for.
- BioEdit (Windows only)
- EMBOSS (OSX/Unix/Linux)
- MEGA4 (Windows/OSX/Linux)
- Gene Construction Kit (Windows/OSX)
- MacVector (OSX only)
- Peaks (Windows/OSX/Linux)
- Unipro UGENE (Windows/OSX/Linux)
Analysis Software geared toward the interests of Evolutionary Analysis, Population Genetics, Phylogenetics and the like:
A List of Phylogeny Programs by Joe Felsenstein If you can’t find what you’re looking for in this comprehensive and exhaustive list, it probably doesn’t exist.
JMATING a software for the analysis of sexual selection and sexual isolation effects from mating frequency data.
Mesquite is software for evolutionary biology, designed to help biologists analyze comparative data about organisms. Its emphasis is on phylogenetic analysis, but some of its modules concern population genetics, while others do non-phylogenetic multivariate analysis.
TREEFINDER is a software to compute phylogenetic trees from molecular sequences.
TreeRot is a very easy program to use for determining Bremer indices (decay indices) for phylogenetic trees. It is also capable of providing partitioned DI, and after your analysis is complete, the program can parse your output log and give you the DI at each node (so you don’t have to go through the log file yourself and do the arithmetic).
SGRunner SG Runner is a graphical user interface for Seq-Gen.
Cadence Cadence helps to conduct Bayesian relative rates tests.
PolypSizer A program for measuring things. Very useful when combined with a digitizing pad and drawing tube.
Gel Reader A program for estimating band sizes on electrophoretic gels.
For Windows:
Acquity 4.0 (ccg12 and ccg13) | MrBayes 3.0b4 |
Clustalx 1.83 | PAUP 4.0 |
DnaSP 4.0 | Phylip 3.65 |
GenePix Pro 6.0 (ccg12 and ccg13) | Phyml 2.4.4 |
Genespring 7.2 (ccg12 and ccg13) | Seq Scape 2.1.1 |
Mega 2 | Sequencing Analysis 5.1.1 |
Mega 3.1 | TreeView |
For Macs:
Clustalx 1.83.1 |
MacClade 4.06 |
MrBayes 3.1.2 |
PAUP 4.0 |
Phylip 3.65 |
Phyml 2.4.4 |
TreeView 1.6.6 |
CoMET (Continuous-character Model Evaluation and Testing) is a Mesquite module for calculating the likelihood of different Brownian motion-based models of continuous character change on phylogenies
Modeltest A tool to select the best-fit model of nucleotide substitution
MrAIC Perl script for selecting DNA substitution models using PHYML
MrModeltest C program for selecting DNA substitution models using PAUP Mailfit: Perl script that runs PAUP and MrModeltest2 (or Modeltest) and sends (optionally) results via e-mail
Burntrees Perl script for manipulating MrBayes tree and parameter files. The script comes with an extra tool, catmb.pl, for concatenating files from separate MrBayes runs.
Dna Editing Software
Vim Nexus syntax highlighting Nexus syntax highlighting files for the Vim editor. Recognizes many/most of the PAUP/MrBayes commands
Vim Nexus syntax highlighting Vim highlighting files for DNA sequence alignments
Free Dna Sequence Analysis Software For Mac Free
MrModelfit/Modelfit Perl script for running MrModeltest2/Modeltest
BootPHYML Perl script for performing non-parametric bootstrapping using PHYML
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DNA Master version 5.0.2 is a 32-bit program for Windows 7, Vista and XP; it will not run on Windows 3.x or DOS machines and may not run on Windows 95, 98, 2000, ME or NT. DNA Master is a freeware. DNA sequence editor and analysis package. I only ask that users register the program and send interested parties here to pick up a version. Thank you for downloading DNA Master from our software library. The download version of DNA Master is 5.22.1. The software is periodically scanned by our antivirus system. We also encourage you to check the files with your own antivirus before launching the installation. The download is provided as is, with no modifications or changes made on.
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How To Download Dna Master On Mac Pro
DNA Master version 5.0.2 is a 32-bit program for Windows 7, Vista and XP; it will not run on Windows 3.x or DOS machines and may not run on Windows 95, 98, 2000, ME or NT. DNA Master is aDna Analysis Tools
freeware DNA sequence editor and analysis package. I only ask that users register the program and send interested parties here to pick up a version. This program is quite powerful in what it can do, and as such does not limit itself in taking up resources. A fast computer with 512 MB of memory (or more) is recommended; greater amounts of memory are required for some functions. The installation of DNA Master requires setting up the Borland database driver subsystem. These files are also used in the Strain Manager, Oligo manager, Chemical Manager, and Invoice Manager programs.*** DNA Master was last upgraded 24 September 2007; this is a major revision and this program will now update itself. This is also a code-breaking update of the original alpha-versions 5.0 and 5.0.1. The auto-update function will replace old versions with a new version that will not be able to access some functions correctly without the database update available in the fresh installation. DNA Master users should uninstall their previous versions before installing the new version of DNA Master; a new installation of the Borland database driver subsystem will be performed.
Slip-stream update: The current version of DNA Master is atleast 2249. If you have an earlier version, and it cannot update itself any longer, that reflects a bug in the FTp subsystem. That bug has been repaired. You will need to downlaod this slip-stream update and unzip it to your DNA master installation directory. After that, the program should be able to update itself once again.